The optogenetic revolution in cerebellar investigations

The cerebellum is most renowned for its role in sensorimotor control and coordination, but a growing number of anatomical and physiological studies are demonstrating its deep involvement in cognitive and emotional functions. Recently, the development and refinement of optogenetic techniques boosted research in the cerebellar field and, impressively, revolutionized the methodological approach and endowed the investigations with entirely new capabilities. This translated into a significant improvement in the data acquired for sensorimotor tests, allowing one to correlate single-cell activity with motor behavior to the extent of determining the role of single neuronal types and single connection pathways in controlling precise aspects of movement kinematics. These levels of specificity in correlating neuronal activity to behavior could not be achieved in the past, when electrical and pharmacological stimulations were the only available experimental tools. The application of optogenetics to the investigation of the cerebellar role in higher-order and cognitive functions, which involves a high degree of connectivity with multiple brain areas, has been even more significant. It is possible that, in this field, optogenetics has changed the game, and the number of investigations using optogenetics to study the cerebellar role in non-sensorimotor functions in awake animals is growing. The main issues addressed by these studies are the cerebellar role in epilepsy (through connections to the hippocampus and the temporal lobe), schizophrenia and cognition, working memory for decision making, and social behavior. It is also worth noting that optogenetics opened a new perspective for cerebellar neurostimulation in patients (e.g., for epilepsy treatment and stroke rehabilitation), promising unprecedented specificity in the targeted pathways that could be either activated or inhibited

Professional competences of pre-service teachers: from the F2F to the online learning programme

The COVID-19 pandemic has created significant challenges for the global higher education community: it has required faculty and students to respond to an unprecedented challenge and to shift suddenly from traditional face-to-face curriculum to distance learning formats through virtual classrooms. Some learning programmes, such as the TFA, had a strong theoretical-practical characterisation and were therefore delimited by regulations requiring full in-presence (F2F) and compulsory attendance. Due to the pandemic, the one-year teaching-learning programme for support teachers (TFA) followed the same path and was redesigned by universities for distance education. The re-design at the University of Macerata was based on pedagogical assumptions: technology was intended as a support to achieve learning outcomes. Our study aims to investigate perceptions of the theoretical and practical skills acquired from the F2F and the online format, considering a group of students enrolled for different school orders who followed both the current online programme and the previous years\u2019 F2F programm

Current status of laboratory and imaging diagnosis of neonatal necrotizing enterocolitis

Necrotizing enterocolitis continues to be a devastating disease process for very low birth weight infants in Neonatal Intensive Care Units. The aetiology and pathogenesis of necrotizing enterocolitis are not definitively understood. It is known that necrotizing enterocolitis is secondary to a complex interaction of multiple factors that results in mucosal damage, which leads to intestinal ischemia and necrosis. Advances in neonatal care, including resuscitation and ventilation support technology, have seen increased survival rates among premature neonates and a concomitant detection in the incidence of this intestinal disease.Diagnosis can be difficult, and identifying infants at the onset of disease remains a challenge. Early diagnosis, which relies on imaging findings, and initiation of prompt therapy are essential to limit morbidity and mortality. Moreover, early management is critical and life-saving.This review summarizes what is known on the laboratory and instrumental diagnostic strategies needed to improve neonatal outcomes and, possibily, to prevent the onset of an overt necrotizing enterocolitis

‘‘Different" bodies: hybridisations and transformations in the biographical storylines of people with disabilities

This article, moving within the conceptual framework of Embodied Cognition (Merleau-Ponty, 1945/2005), aims at investigating the embodied representations of the bodies of people with physical disabilities in identity construction processes. In this way, the article helps to shape a culture that embraces differences and values body diversity. As described by existing literature on the subject (Clandinin & Connelly, 2004; Giaconi, Del Bianco, D’Angelo, Taddei, Caldarelli & Capellini, 2021; Giaconi, Del Bianco, D’Angelo, Taddei & Rodrigues, 2020), the life stories of people with disabilities, including athletes, represent a privileged way to access the processes of signification of corporeity, interpretation of diversity and overcoming of binary logics (male/female, natural/artificial). Indeed, when the hybridisations and transformations of “different” bodies emerge directly from the words of people with disabilities, one can gauge their profound distance from media representations of disability, awash with ableist narrative styles (Bocci, De Castro & Zona, 2020; Giaconi & Capellini, 2019). Following this direction, this article will highlight the possibilities of a pedagogical work intentionally focused on the narratives of athletes with disabilities

Global Calculations of Density Waves and Gap Formation in Protoplanetary Disks using a Moving Mesh

We calculate the global quasi-steady state of a thin disk perturbed by a low-mass protoplanet orbiting at a fixed radius using extremely high-resolution numerical integrations of Euler's equations in two dimensions. The calculations are carried out using a moving computational domain, which greatly reduces advection errors and allows for much longer time-steps than a fixed grid. We calculate the angular momentum flux and the torque density as a function of radius and compare them with analytical predictions. We discuss the quasi-steady state after 100 orbits and the prospects for gap formation by low mass planets.Comment: Accepted to Ap

Effects of compost-bedded pack barn on circulating cortisol and beta-endorphins in dairy cows: A case study

SIMPLE SUMMARY: Management and housing conditions have been reported to significantly affect the health and welfare of livestock species. Therefore, the adoption of novel, alternative housing systems (such as the compost-bedded pack barn, developed in the USA) requires extensive research to assess the implications for animal welfare. From a general point of view, animal welfare is typically assessed by means of animal-based (such as blood biochemical markers) and resource-based (such as management practices) indicators. Based on such considerations, the present study evaluated the fluctuation of circulating cortisol and beta-endorphins in dairy cows housed in a conventional freestall barn (FB) and in the alternative compost-bedded pack barn (CB). The results obtained suggest that the CB housing system did not elicit significant changes in either blood cortisol or beta-endorphins. ABSTRACT: The up-to-date literature suggests that the compost-bedded pack barn housing system is capable of remarkably improving productive and reproductive performance, as well as health status and welfare, in dairy cattle. However, there is currently limited knowledge available on the endocrine and biochemical changes in animals housed in such alternative systems. Therefore, this study aimed to measure blood cortisol (COR) and beta-endorphins (BE) in 22 two-year-old primiparae Fleckvieh cows, who were randomly allotted to the following two different housing systems: CB (n = 11) and FB (n = 11). Blood samples were collected at the beginning of the experiment (T0) and every two months thereafter (T1, T2, and T3). The COR and BE were measured through an immunoenzymatic kit. With the only exception being T0, no differences were observed over time between the two groups, neither for COR nor for BE. However, the blood cortisol levels of the CB cows decreased over time, while a T1 peak was identified in the FB group. On the contrary, both the housing systems displayed numerically higher BE at T3 than at the other experimental times. Therefore, the overall data suggest that the compost-bedded pack barn did not significantly affect the studied parameters. Accordingly, cow welfare should be assessed using a wider panel of animal-based indicators

Final results of an experiment to search for 2beta processes in zinc and tungsten with the help of radiopure ZnWO4 crystal scintillators

A search for the double beta decay of zinc and tungsten isotopes has been performed with the help of radiopure ZnWO4 crystal scintillators (0.1-0.7 kg) at the Gran Sasso National Laboratories of the INFN. The total exposure of the low background measurements is 0.529 kg yr. New improved half-life limits on the double beta decay modes of 64Zn, 70Zn, 180W, and 186W have been established at the level of 10^{18}-10^{21} yr. In particular, limits on double electron capture and electron capture with positron emission in 64Zn have been set: T_{1/2}(2\nu 2K) > 1.1 10^{19} yr, T_{1/2} (0\nu 2\epsilon) > 3.2 10^{20} yr, T_{1/2} (2\nu \epsilon \beta^+) > 9.4 10^{20} yr, and T_{1/2} (0\nu \epsilon \beta^+) > 8.5 10^{20} yr, all at 90% C.L. Resonant neutrinoless double electron capture in 180W has been restricted on the level of T_{1/2} (0\nu 2\epsilon) > 1.3 10^{18} yr. A new half-life limit on alpha transition of 183W to the metastable excited level 1/2^- 375 keV of 179Hf has been established: T_{1/2} > 6.7 10^{20} yr.Comment: This is an author-created, un-copyedited version of an article published in J. Phys. G: Nucl. Part. Phys. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The definitive publisher authenticated version is available online at doi: 10.1088/0954-3899/38/11/11510

The Pale-breasted Thrush (Turdus leucomelas) preys on a gekkonid lizard and an anomalepidid snake

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)193450452Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

The rna-binding ubiquitin ligase mex3a affects glioblastoma tumorigenesis by inducing ubiquitylation and degradation of rig-i

Glioblastoma multiforme (GB) is the most malignant primary brain tumor in humans, with an overall survival of approximatively 15 months. The molecular heterogeneity of GB, as well as its rapid progression, invasiveness and the occurrence of drug-resistant cancer stem cells, limits the efficacy of the current treatments. In order to develop an innovative therapeutic strategy, it is mandatory to identify and characterize new molecular players responsible for the GB malignant phenotype. In this study, the RNA-binding ubiquitin ligase MEX3A was selected from a gene expression analysis performed on publicly available datasets, to assess its biological and still-unknown activity in GB tumorigenesis. We find that MEX3A is strongly up-regulated in GB specimens, and this correlates with very low protein levels of RIG-I, a tumor suppressor involved in differentiation, apoptosis and innate immune response. We demonstrate that MEX3A binds RIG-I and induces its ubiquitylation and proteasome-dependent degradation. Further, the genetic depletion of MEX3A leads to an increase of RIG-I protein levels and results in the suppression of GB cell growth. Our findings unveil a novel molecular mechanism involved in GB tumorigenesis and suggest MEX3A and RIG-I as promising therapeutic targets in GB